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Integrated Staining and Sealing Fume Hood: Principles, Features, and Applications
Created on 08.19
Integrated Staining and Sealing Fume Hood: Principles, Features, and Applications
A Comprehensive Guide to Definition, Operation, Benefits, and Maintenance
I. Definition and Overview
An Integrated Staining and Sealing Fume Hood is a specialized laboratory ventilation system designed to combine the processes of staining (染色, staining treatment of materials or biological samples) and sealing (封闭, protective or containment sealing) within a single enclosed structure. It ensures a controlled environment by providing local exhaust ventilation (LEV), preventing hazardous fumes, vapors, or particulates from escaping into the laboratory.
Key terminology:
- HEPA (High-Efficiency Particulate Air) Filter
- ULPA (Ultra-Low Penetration Air) Filter
- Airflow Velocity
- ISO 14644-1: Cleanrooms and Associated Controlled Environments
II. Working Principle
The fume hood operates based on a controlled airflow circulation system:
- Air Inflow
- Filtration System
- Exhaust and Recirculation
- Cross-Contamination Prevention
- Automatic Control System GMP (Good Manufacturing Practice)
III. Main Functions
- Minimizes cross-contamination risk between processes.
- Maintains a sterile and clean working environment.
- Protects operators from chemical exposure and harmful vapors.
- Enhances productivity by integrating two processes into one system.
- Ensures compliance with ISO 14644-1 FDA cleanroom requirements
IV. Structural Features and Materials
The integrated fume hood is manufactured using durable and easy-to-clean materials:
- Stainless steel (SS304/SS316) – corrosion-resistant, hygienic.
- Powder-coated cold-rolled steel – robust structural support.
- Aluminum alloy frames – lightweight yet strong.
- Tempered glass – chemical resistant and transparent viewing.
- Seamless arc-shaped corners – prevent dust accumulation and allow easy cleaning.
V. Classification and Technical Parameters
Model | Airflow (m³/h) | Power (W) | Filtration Level | Dimensions (mm) |
ISFH-900 | 1000 | 550 | G4 + H13 HEPA | 900 × 700 × 2200 |
ISFH-1200 | 1500 | 750 | G4 + H14 HEPA | 1200 × 800 × 2200 |
ISFH-1500 | 2000 | 900 | G4 + ULPA | 1500 × 800 × 2200 |
VI. Typical Application Industries
The integrated staining and sealing fume hood is widely applied in:
- Cleanroom Engineering
- Medical Engineering
- Construction & Architectural Engineering
- Laboratory Engineering
- Medical Devices & Equipment Manufacturing
- Pharmaceutical and Biotech Facilities
- Environmental Protection & Green Technology
VII. Installation and Usage Considerations
- Must comply with GMP standards ISO 14644-1
- Install in areas with stable airflow, avoiding direct drafts from doors or HVAC vents.
- Ensure proper electrical grounding and compliance with safety codes.
- Routine calibration of airflow velocity monitors is recommended.
VIII. Maintenance and Care
- Regularly clean and disinfect all interior surfaces.
- Replace pre-filters every 3–6 months; HEPA/ULPA filters every 12–18 months (depending on usage).
- Check and recalibrate control systems and airflow monitors.
- Lubricate mechanical parts and inspect seals to ensure airtightness.
- Maintain detailed service logs for regulatory compliance.
IX. Advantages
- Integration of staining and sealing reduces equipment footprint.
- Improved operator safety through advanced containment systems.
- Energy-efficient design lowers operational costs.
- Modular structure allows customization for different industries.
- Long service life with minimal maintenance requirements.
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